MODEL THETA — 3D video microscopy

Model Theta is the worlds fastest single molecule sensitive 3D video microscope. With a field-of-view covering 10,000 cells in high resolution, in 3D and at a frame rate of 40 Hz, SINGLE takes imaging and high content screening to a whole new level.


Single Technologies Model Theta is the worlds fastest single molecule sensitive 3D video microscope. It allows you to make high resolution videos of molecules, live cells and tissue. Model Theta is capable of producing 10,000 3D images of single cells in just 1 second. We call it video-based High Throughput Imaging.

— 10,000 3D images of single cells in just 1 second —


Along with its speed and true 3D optics, Single Technologies Model Theta is equipped with a unique Dynamic Imaging mode. This enables it to track individually labelled molecules, biomarkers, protein-protein interactions, protein complexes or vesicles within each living cell or well without any amplification. This facilitates extremely simple, accurate, novel and flexible assays. If the cells are fixed or you are happy with your existing cell-based or biochemical assay, then just select the Static mode for High Throughput Imaging.


Due to the ultra-sensitive detection system of Model Theta, it is possible to measure on extremely diluted samples or sub-microlitre sample volumes, saving both reagent and compound costs.

Delivery estimate for new orders is fall 2016.



Scanning System

  • Resolution: 0.3x0.5x0.7 um
  • Scanning field: 65x125 mm
  • Scanning depth: <100 um

Scanning Speed

  • 65x125 mm in 220 s (@ 0.5 um resolution)
  • 65x125x0.01 mm 3D images in 1.2 h


  • Default excitation wavelengths (nm): 450, 525, 638 nm. Optional wavelengths: 375, 405, 478, 780 (2-photon excitation)

Detection system

  • Single photon sensitive camera


  • Molecules
  • Cells
  • Tissue


  • Various plate designs, from single well (large samples) to 1536 well plates


  • User friendly GUI to control Model Theta
  • Open microscopy (OME), Image J, Matlab and Python compatible 5D file format
  • FCS and STICS software


  • Motorized fast-change filter wheel (6 filters)
*This specification is preliminary and changes may arise during the development phase of Model Theta