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ISS

in situ Sequencing

 

in situ Sequencing

In situ RNA seq is a new method of sequencing a cells's RNA while it remains in tissue or culture using next-generation sequencing. Compared with other approaches histological context and spatial information are preserved, and In-situ RNA seq has the potential to transform several areas of research, including drug development in many diseases and characterizing all the possible cells states in the body.

Single is collaborating with SciLifeLab, one of the leading institutions pioneering the development of in situ RNA seq. The fast 3D imaging of large areas without compromising single molecule sensitivity and diffraction limited resolution enables the method to reach its full potential, and the capabilities of the fluidic system streamlines the chemistry

 
 

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Common applications when localization matters

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Localization of cell types in organs

With a detailed understanding of brain cell types, and their localization, comes an opportunity to develop new molecular pathology to diagnose and understand brain disease

tumour heterogenity

Tumours comprise a heterogeneous collection of cells with distinct genetic and phenotypic properties that can differentially promote progression, metastasis and drug resistance. Profile individual cells within tumours and investigate what roles they play in these processes. 


Workflow

Left: add tissue to our flow cells and plug it into the Theta Sequencer. Right: locations and sequencing data generated.

Left: add tissue to our flow cells and plug it into the Theta Sequencer. Right: locations and sequencing data generated.


Learn more about the Theta Sequencer