FREQUENTLY ASKED QUESTIONS


General

WHAT ARE THE BENEFITS WITH SINGLE MOLECULE SENSITIVITY?

Single molecule sensitivity means that the instrument can detect concentrations down to one single fluorescent molecule within the sample. This contrasts with other instruments which requires high concentrations or other means of amplification to distinguish the molecules from random noise.  .


IS THE MEASUREMENTS ONLY PERFORMED ON ONE CELL/MOLECULE?

No, data is collected from the whole sample.

WHY SINGLE CELLS AND NOT AVERAGING?

Because analyzing each individual cell improves the quality of the assay. A cell population is almost without exception heterogeneous, hence highly expressive and dead cells will bias the results when averaging. Of course it is still possible to average over all cells.


AREN’T SINGLE MOLECULE AND SINGLE CELL MEASUREMENTS TOO SLOW FOR INDUSTRIAL APPLICATIONS?

Not anymore, Model Theta turns the world upside down.


— AREN’T SINGLE MOLECULE AND SINGLE CELL MEASUREMENTS TOO SLOW FOR INDUSTRIAL APPLICATIONS?

Not anymore, Model Theta turns the World upside down.—


CAN I USE THE SAME PLATES FOR CELLS, BIOCHEMICAL REACTIONS AND TISSUE?

The sample plates can be used for cells and biochemical reactions but not for tissue. Standard format sizes of microtiter plates are available (8-, 96-, 1536-wells), but Single plates are required.

PLATES


WHAT ABOUT LABELING?

It is of course important that the labeling doesn’t affect the biological activity. Thanks to the high sensitivity and the rapid development of novel labeling techniques and affinity molecules, this risk is heavily reduced. Model Theta possesses both fluorescence and label free detection.

LABELING


SERVICES

WHAT IF SINGLE DOESN’T DELIVER IN A SERVICE AGREEMENT?

Then we will not charge you (unless we previously agreed otherwise).

COULD A SMALL BIOTECH OR INDIVIDUAL RESEARCHER TAKE ADVANTAGE OF THE SERVICES?

Yes, we welcome anyone. You can take advantage of the technology without buying the equipment by utilizing our services – see Services.


Don't hesitate to send us your questions at faq@singletechnologies.com